In this section
The principle of the semen analysis is to assess the macroscopic,
and microscopic, quantitative and qualitative, features of an ejaculate. The
former examines components; liquefaction, viscosity, appearance, volume and pH.
The latter provides an analysis of sperm concentration, sperm motility, sperm
velocity, sperm morphology and sperm viability. Other notable elements of a
semen analysis include the assessment of leukocytes and prominent morphological
defects that may be of a genetic origin.
The Sperm Mar test (MAR) enables the detection of sperm-bound
antibodies of immunoglobulin classes: IgG and IgA. The MAR can be used either
as a "direct" test for detecting antibodies bound to a patient's
spermatozoa in seminal fluid, or as an "indirect" test for detecting
sperm antibodies in serum or various reproductive tract fluids.
Fructose is qualitatively assessed for presence or absence in
seminal plasma using a simple colour change test. The Seliwanoff assay is
utilised for this test.
Excessive reactive oxygen species (ROS), produced by both
spermatozoa and leukocytes, are normally eliminated effectively by seminal
plasma free radical antioxidant scavengers and antioxidant enzymes. Assessing
the electropotential of seminal fluid (mV/million sperm/mL) enables the
detection of an imbalance in this biological mechanism, and identifies semen
samples exhibiting Oxidative Stress.
laboratory is currently performing validation work for this test and it will
soon be available as a diagnostic test.