16S rRNA Gene Nucleic Acid Detection


Test Name
16S rRNA Gene Nucleic Acid Detection
Test Code
16SPC
Specimen Type

Tissue Biopsies

EDTA Blood

CSF

Minimum Volume
0.5 ml Fluid, Tissue
Preferred Volume
1ml Fluid, Tissue
Comments

Testing will only be performed after discussion with Clinical Microbiologist.

Only samples from aspetic sites should be tested.  The presence  of more than one type of organism will either result in and unassesable sequence or only the predominant organism being sequenced.

Colonies on non selective agar plates (or one colony in 200ul PBS) may be forwarded from the bacteriology Laboratory.  Bacterial cultures older than 24 hours must be placed at 4 deg C until tested.  Colonies older than 72 hours should not be tested.

Storage

4 deg C up to 3 days

-20 deg C 3-10 days

Transport

4 deg C

Assay Performed
Molecular Microbiology
RCH
Level 4, 50 Flemington Rd, Parkville 3052
Laboratory Hours: 0845-1715 Mon-Fri
No after hours service; if urgent testing is required, the requesting clinician is to contact on-call Clinical Microbiologist for advice via switchboard
9345 4673
Assay Frequency

Weekly as requested

ABO Titre